DNA methylation changes in shoot and root at seedling stage under non-stress and salinity stress conditions.
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aMSAP (%) = [(II+III+IV)/(I+II+III+IV)]×100;bFully methylated bands (%) = [(III+IV)/(I+II+III+IV)]×100;cHemimethylated bands (%) = [(II)/(I+II+III+IV)]×100.Type I indicated absence of methylation due to the presence of bands in both EcoRI/HpaII and EcoRI/MspI digest; type II bands appeared only in EcoRI/HpaII digestion but not in the EcoRI/MspI digest; type III generated bands obtained in EcoRI/MspI digest but not in the EcoRI/HpaII digest; and type IV represents the absence of band in both enzyme combinations.
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2015-12-02



