Hyaluronidase activity of sTMEM2 (Filtration)
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资源简介：
Ultrafiltration (molecular weight cut-off 50 kDa) of 0.1 μg/ml fluorescein-labelled high-molecular-weight hyaluronan (HMW-HA, ~800 kDa, Carbosynth) incubated overnight with 100 μg/ml transmembrane protein 2 ectodomain (sTMEM2) or sperm hyaluronidase (HAase, Sigma-Aldrich, H3884). The reaction buffer was 100 mM MES, pH 6.0, 1 mM CaCl2. The mock condition contained an irrelevant protein at 100 μg/ml (Endo180 domains 1-4).
创建时间：
2023-01-30
