Microbial community of the rhizosphere of Arabidopsis with different small RNA levels

1)We treated Arabidopsis thaliana Col-0 with 500 uL of water (control condition) or a miPEP solution (20 uM of miPEP159a, miPEP159b, or miPEP159c), applied at the base of the crown, 3 times a week for a total of 10 applications. We then extracted the DNA from the roots and the attached rhizosphere and sequenced and quantified the 16S RNA gene2)Five A. thaliana mutants were chosen: RTL1, RTL1myc, Ago1-27, Dcl1-2, and Hen1-4. The plants were grown for a month, after which the roots and attached rhizosphere were sampled, its DNA extracted, amplified using 16S rRNA gene primers, and sequenced.