Gene expression changes in mouse kidney cancer cells (RENCA) after sunitinib resistance and acute and chronic withdrawal

Transcriptome analysis was used to identify changes in mouse kidney cancer cells (RENCA) resistant to sunitinib (SuR) and after short and long term sunitinib withdrawal in vitro (ST-W for 48 hours and LT-W for more than 2 months, respectively). Mouse kidney cancer cells (RENCA) resistant to sunitinib were derived in vivo after continuous treatment with 60mg/kg/day sunitinib from post-surgical metastatic lesions. Resistant cells were maintained in vitro with 5μM sunitinib for more than 2 months.