LPS-stimulated GM-CSF and M-CSF polarised human monocyte derived macrophages

Monocyte derived macrophages (MDMs) were generated from monocytes isolated from peripheral blood mononuclear cells using a Percoll gradient and adherence technique, followed by culture for 12 days with either GM-CSF to generate G-Mf (GM-CSF polarised macrophages) or M-CSF to generate M-Mf (M-CSG polarised macrophages). MDMs were then stimulated for 4h with/without 1 µg/ml LPS.