Figure S1 - Steric Shielding of Surface Epitopes and Impaired Immune Recognition Induced by the Ebola Virus Glycoprotein

EBOV GP masks multiple epitopes on MHC1. OV79 SL9 target cells were mock transduced or transduced with Adenoviral vectors expressing GFP (Ad GFP) or GFP and EBOV GP (Ad GP) at an MOI of 300. 48 h after transduction, cells were indirectly stained for different epitopes on MHC1 with primary antibody clones W6/32, YTH862.2, BB7.2 and GJ14 and detected with Alexa Fluor 647-conjugated secondary antibodies; isotype antibody =  grey peak; mock transduction =  blue trace; Ad GFP =  green trace; Ad GP =  orange trace. The approximate location of each epitope is marked by the yellow star in a cartoon depiction of MHC1 to the right of each graph. The W6/32 clone recognizes the MHC1 heavy chain and the β2 microglobulin. The YTH862.2 clone recognizes the α1 domain of the MHC1 heavy chain. The BB7.2 clone is specific for HLA-A2 and recognizes the α2 domain of the heavy chain. The GJ14 clone recognizes the β2 microglobulin.
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