Acute treatment with kynurenic acid in inguinal primary adipocytes

Tryptophan-kynurenine metabolism plays an important role in the pathogenesis of several psychiatric diseases but its physiological function in peripheral tissues remains unclear. Physical exercise training activates a biochemical pathway in skeletal muscle that protects from neuroinflammation and, as a byproduct, leads to kynurenic acid accumulation in the periphery. We therefore investigated the effects of kynurenic acid in murine white adipose tissue. We used microarrays to detail global programme of gene expression underlying Kynurenic acid mediated effects on inguinal primary adipocytes. Fully differentiated inguinal primary fat cells were treated for 12h with 50μM of kynurenic acid or PBS (control), in triplicate. RNA was extracted and used for global gene expression analyses.