Thermodynamic and physicochemical characteristics of candidate primer pairs (N1–N3) designed in silico for amplification of the eNOS (endothelial nitric oxide synthase) gene from Rattus norvegicus mRNA

Thermodynamic and physicochemical characteristics of candidate primer pairs (N1–N3) designed in silico for amplification of the eNOS (endothelial nitric oxide synthase) gene from Rattus norvegicus mRNA (Accession: NM_021838.2), including primer sequence, length, melting temperature (Tm), predicted hairpin Tm, GC content, self 3′-complementarity, and expected amplicon size.