NUDT1 hydrolyzes O6-methyl-dGTP to O6-methyl-dGMP

NUDT1 (MTH1) catalyzes the reaction of O6-methyl-dGTP and water to form O6-methyl-dGMP and PPi (pyrophosphate) (Jemth et al. 2018). Four NUDT1 proteins have been identified, encoded by a single gene with alternative start codons (Oda et al. 1999). The shortest of these, NUDT1 p18, has been shown to be enzymatically active as a monomer associated with a magnesium ion (Mishima et al. 2004; Sakumi et al. 1993; Takagi et al. 2012). The longer isoforms all consist of the p18 polypeptide with aminoterminal extensions and are presumed to be active as well but have not been experimentally characterized. The p18 isoform is predominantly cytosolic (Kang et al. 1995).

NUDT1（MTH1）催化O6-甲基-dGTP与水反应生成O6-甲基-dGMP和焦磷酸（PPi）（Jemth等，2018年）。已鉴定出四种NUDT1蛋白，这些蛋白由单一基因编码，具有不同的起始密码子（Oda等，1999年）。其中最短的NUDT1 p18已被证实作为一种与镁离子结合的单体酶具有催化活性（Mishima等，2004年；Sakumi等，1993年；Takagi等，2012年）。较长的同源变体均由p18多肽组成，并在氨基末端具有延伸部分，据推测也具有活性，但尚未通过实验进行特征化。p18同源变体主要存在于细胞质中（Kang等，1995年）。