Effect of BXL-01-0029 onto IFNγ-treated Hfsmc.

A, Western blot analysis to assess Stat-1 activation after 12 min stimulation with IFNγ (1000 U/ml), in presence or absence of BXL-01-0029 (10−8 M) showed that IFNγ increased Stat-1 phosphorylation, whereas BXL-01-0029 did not exert any effect (upper A); total Stat-1 was used as loading control (middle A). The densitometric analysis is reported in lower A (*P<0.05 vs. control) and results are expressed as ratio phosporylated/total protein arbitrary units vs. IFNγ-treated cells, taken as 1 (mean±S.E.). B, Cell incubation for 24 h with BXL-01-0029 (10−8 M) did not affect IFNγ-induced CXCL10 secretion, taken as 100%. Results (mean±S.E.) are derived from five experiments, using distinct cell preparations.