Spheroids, after being formed, were subjected to treatment with radiocontrasts for 24 hours and the antioxidant NAC. Then, the oxidation of the H2DCFDA probe was quantified by fluorescence analysis in
Sample multiplexing in flow cytometry is a powerful technique which allows for reduction of error, inclusion of control samples for batch effect correction, and reduction in both time and consumable u
Intracellular ROS formation was measured using flow cytometry in yeasts loaded with H2DCFDA. Samples were treated with APCP for 2 minutes and analyzed at 30, 60 and 120 seconds after treatment. Data a
