Single-cell RNA sequencing for heart failure heart of mice

Heart failure (HF) is the ultimate outcome of most cardiovascular disorders with high prevalence and poor prognosis. The transverse aortic constriction (TAC) method is currently the most widely utilized approach for investigating HF induced by left ventricle pressure overload. We explore potential genes with significant changes in HF through single-cell sequencing. Overall design: Samples were prepared from uninjured mice, as well as from mice which underwent TAC operation 6 weeks to model pressure overload injury. Hearts were dissociated into single cells. We combined single-cell suspensions from 3-5 hearts into one sample.