K27M and K36M mutations on non canonical Histone 3.3 promote redistribution of antagonistic chromatin marks, disrupted development, and tumorigenesis

We conducted RNA-seq analysis to identify genes that are differentially expressed in H3K27M and H3K36M oncohistones compared to wt and control eye tissue (eye discs), followed by smRNA-seq profiling based on our results and to confirm upregulation of piRNAs 3 data types (RNA-seq, smRNA-seq and ChIP-seq data). RNA-seq and smRNA-seq experiments has 12 samples, which is composed of 3 replicates of 4 genotypes (H3K27M, H3K36M, histone WT and control eye tissue (Yw)). Additional RNA-seq RNAi experiments has been performed on 10 sets (3 replicates each, 30 samples total). H3K27me3 and H3K36me2 ChIP-seq was also performed (14 samples)