Genomic sequences of the carabid beetles Pseudamara arenaria (LeConte, 1848) and Miquihuana rhadiniformis Barr, 1982

Miquihuana rhadiniformis Barr, 1982 and Pseudamara arenaria (LeConte, 1847) are enigmatic North American carabid beetles whose tribal-group placement has been in doubt. As these species are relatively rarely collected, neither Miquihuana Barr, 1982 nor Pseudamara Lindroth, 1968 have been sampled in previous phylogenetic studies and attempts to obtain DNA sequences through standard PCR of available specimens failed. We used Illumina sequencing to recover nuclear gene fragments (18S rDNA, 28S rDNA, wingless, and CAD) from pinned M. rhadiniformis and 70%-ethanol-preserved P. arenaria. Combining these data with data from exemplar taxa from putatively closely related tribal-level clades, we examine the tribal relationships of Miquihuana and Pseudamara.