Naïve Regulatory T Cell Subset Is Altered in X-Linked Agammaglobulinemia (part2)

Clonosets from 3 cell subsets (CD4+ mature naive, CD4+ RTE, naive Treg) sorted from blood of XLA and healthy (old and young) donors. Total RNA was isolated using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. In all experiments described here, cDNA libraries were obtained using 5’-RACE with unique molecular identifiers (UMI) using Human TCR profiling kit (MiLaboratory LLC). Libraries were sequenced on Illumina NextSeq 500 using 300 cycle reagent kit, paired-end 150 + 150 nt mode. Clonotypes were inferred from reads using MIGEC (UMI threshold = 2) + MIXCR + VDJTOOLS