RNA-seq identification of herpes simplex virus type 1 transcription in Neuro-2a cells overexpressing FOXK1 and FOXF1

We used inducible Neuro-2a cell lines with stable expression of FOXK1 and FOXF1. The cells were induced to express FOX protein under doxycycline for 36 hours and then infected with HSV-1 for five hours. The cells were collected and cleaved, and total RNA was extracted for RNAseq analysis Overall design: Through RNAseq analysis, we mainly focused on the changes in the number of viral reads under the condition of FOX protein overexpression. P stands for wild-type Neuro-2a cells, K stands for Neuro-2a cells that overexpress FOXK1, and F stands for Neuro-2a cells that overexpress FOXF1.