Replication Data for: A retrospective analysis of P. falciparum drug resistance markers detects an early (2016/17) high prevalence of the k13 C469Y mutation in asymptomatic infections in Northern Uganda

This data were generated from targeted amplicon sequencing of Plasmodium falciparum, the most lethal malaria parasite affecting humans. The sequencing targeted the following P. falciparum genes: apical membrane antigen 1 (Pfama1: PF3D7_1133400), dihydrofolate reductase (Pfdhfr: PF3D7_0417200), dihydropteroate synthase (Pfdhps: PF3D7_0810800), kelch13 (Pfk13: PF3D7_1347700), and multidrug resistance 1 (Pfmdr1: PF3D7_0523000). These genes are critical for understanding the parasite's resistance mechanisms and for the development of antimalarial drugs. The samples were collected from patients at Kitgum General Hospital, located in Uganda, between September 2016 and September 2017. The collection aimed to investigate the genetic diversity and drug resistance patterns of P. falciparum in this region. Sequencing libraries were prepared from the collected samples and were normalized to equimolar concentrations to ensure uniform coverage across all targeted regions. The libraries were then denatured and spiked with an 8% PhiX DNA control to monitor sequencing performance. Sequencing was performed on an Illumina MiSeq platform using the MiSeq Reagent Kit v3, achieving read lengths of 2x300 base pairs.