Transcription profiling by array of human primary melanoma samples with distinct biological parameters to compare their gene expression pattern

Comparison of gene expression patterns between primary melanomas with distinct biological parameters: our study applied different experimental factor types for comparisons, namely tumor surface ulceration, metastasis formation, and disease stage by Clark level and Breslow thickness. Our aim was to describe molecular signalling of primary melanomas based on differently expressed genes and to identify experimental factors that influenced by differently expressed genes. This assessment led us to map the most prevalent alterations associated with this clinical parameter.Out further aim was to conduct an integrated gene expression and copy number analysis combining our results from Affymetrix and NimbleGen microarrays. The main purpose of this part of the study was to give functional relevance to the copy number events by providing a statistically powerful integrated interpretation of gene expression changes and copy number alterations.