A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathway
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE210768
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To identify APA changes in Tsc1-cKO mice, cTag-PAPERCLIP was performed on pAAV-Camk2a-iCre injected Tsc1-WT and Tsc1-floxed cTag-PABP mice. To identify CPSF6-dependent PAS, PAPERCLIP was performed on shCPSF6-BE2C cells with/without doxycycline treatment. cTag-PAPERCLIP and PAPERCLIP library construction was performed as previously described (Hwang et al., Neuron 2017; Kunisky et al., Cell Rep 2021) and the libraries were sequenced on Illumina NextSeq
创建时间：
2023-09-05
