Reprogramming of ovarian aging epigenome by resveratrol [RNA-seq]

Depletion of oocytes and follicles and reduced oocyte quality contribute to age-associated ovarian senescence and infertility. Telomere shortening and altered methylation make two major contributions to general aging. Resveratrol acts as anti-oxidant and Sirt1 activator to alleviate aging including reproductive aging. It remains elusive whether resveratrol can reprogram the aging epigenome. We sought to examine aging ovarian epigenome and the potential effects of resveratrol by combined analysis of telomere length, transcriptome and methylome mainly in oocytes and also in granulosa cells, two major cell types in the ovary. for methylation analysis, 2~3 repeats of mouse MII oocytes, 10 oocytes each sample； for RNA-Seq analysis, ≥3 repeats of mouse MII oocytes, 1 oocyte each sample； for RNA-Seq analysis, 3 repeats of mouse granulosa cells(GCs) , 500 GCs each sample. "OKOC"for old age(10-month-old) Tet2 knockout control group "OKOR"for old age(10-month-old) Tet2 knockout resveratrol treated group "OWTC"for old age(10-month-old) WT control group "OWTR"for old age(10-month-old) WT resveratrol treated group "YKO"for young(2-month-old) Tet2 knockout control group "YWT"for young(2-month-old) WT control group "MWTC"for middle-age(6-month-old) WT control group "MWTR"for middle-age(6-month-old) WT resveratrol treated group "OKOC"for old age(10-month-old) Tet2 knockout control group "OKOR"for old age(10-month-old) Tet2 knockout resveratrol treated group "OWTC"for old age(10-month-old) WT control group "OWTR"for old age(10-month-old) WT resveratrol treated group "YKO"for young(2-month-old) Tet2 knockout control group "MKOC"for middle age(6-month-old) Tet2 knockout control group "MKOR"for middle age(6-month-old) Tet2 knockout resveratrol treated group old age(10-month-old) resveratrol treated group old age(10-month-old) control group middle age(6-month-old) control group