Newly defined human hematopoietic stem cell populations

Transcriptomic profiling (RNA-seq) of primitive human progenitor populations CD34+CD38-CD45RA-CD90-EPCR-; hereafter CD90-EPCR- MPP. Purpose: To characterize the transcriptomic profile of the human CD90-EPCR- MPP progenitor population. Methods: Cells were obtained from human cord-blood and flow-sorted using the surface antigens described. 5 CD90-EPCR- biological replicates were used to extract total RNA and quality was verified (RIN >8). Libraries were prepared using 20-25 ng/sample of starting RNA. All samples were sequenced two independent times. Conclusions: This study represents the analysis of transcriptomes from a human progenitor population, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles of other human blood stem/progenitor populations.