Single cell gene expression profile of irradiated small intestinal crypt cells of WT and CREPT KO mice

To investigate the role of CREPT in intestinal regeneration, we performed single cell RNA sequencing (scRNA-seq) analyses on irradiated small intestinal crypt cells of WT and CREPT KO mice to figure out the change of different types of intestinal epithelial cells. CREPT was specifically deleted in intestinal epithelial cells with Villin-cre. Small intestinal crypts were isolated from irradiated WT and CREPT KO mice at 3 and 5 days post irradiation (dpi). Viable single cells were sorted by fluorescence-activated cell sorting (FACS) and subjected to scRNA-seq.