Time-lapse RNA-seq analysis to study the gene networks in cell fate transition in de novo root regeneration from detached Arabidospis leaves

Detached Arabidopsis leaves can regenerate adventitious roots, providing a platform to study de novo root regeneration (DNRR). We performed time-lapse RNA-seq within 5 d revealed activation of gene networks in cell fate transition. Overall design: We used the adventitious rooting assay from detached Arabidopsis leaves in this study. We performed time-lapse RNA-seq analysis of the wounded region of leaf explants at t0, 6 h, 12 h, 1 d, 2 d, 3 d, 4 d, and 5 d after detachment to study the gene networks in cell fate transition.