Trypanosoma cruzi strain:strain Y and CL-Brener Targeted Locus (Loci)

The fact that almost all individual coding genes in trypanosomatids have no promotors for RNA pol II point out that the gene expression regulation in these organisms is mainly post-transcriptional. Therefore, the mRNA processing coul be an important point to control the genetic expression of protein coding genes. All pre-mRNA in trypanosomatids must be processed into units in order to be exported from the nucleus and translated into proteins. The process of trans-splicing and polyadenylation are responsible for processing all trypanosomatids mRNA and generate different isotypes from a simple gene. Indeed, events of trans-splicing and alternative polyadenylation produce the UTRs and defined their sequence composition that has a major role in the, mRNA turnover, mRNA exporting to the cytoplasm and mRNA translation. Little is known regarding the RNA processing of multi-copy genes in different stages of trypanosomatids. Since T. cruzi stages face dissimilar environmental conditions, the knowledge of how multicopy genes are processed in such conditions would help to understand the role of this process in generating genetic diversity that contribute to the parasite survival. We used calmodulin gene as a model ought to its essential role in many physiological processes of T. cruzi.