Targeting individual cells by barcode in pooled sequence libraries. Targeting individual cells by barcode in pooled sequence libraries

Purpose: Selectively sequence desired single cells from pooled single cell libraries Overall design: 10X libraries were prepared from two FACs sorted populations (HLA-DR and CD19) followed by enrichment for targeted single cells in triplicate. Enrichment was achieved with multiplex PCR with primers targeting the single cell barcodes that are added through library preparation. NOTE: Submitter states that the raw data will be submitted to dbGaP (https://www.ncbi.nlm.nih.gov/gap) because of patient privacy concerns.