Gene changes associated with deletion of Esrrg in Dopaminergic neurons in mice [H202SC21050050]

Purpose: The goal of this study is to understand what gene changes are associated with removal of Esrrg in dopaminerigc neurons from the mouse midbrain using BAC-TRAP and an AAV for Thcre Methods: Midbrain from mice expressing the L10a transgene used for BAC-TRAP method of RNA isolation both with and without deletion of Esrrg using AAV:THCre were aged 1 month post-injection and midbrains were then extracted and flash frozen on dry ice. RNA was isolated using the BAC-TRAP method and samples were sent for sequencing. Overall design: Midbrain mRNA profiles of mice 1 month post deletion of Esrrg in the midbrain compared to mice with no Esrrg deletion. All mice were expressing the L10a transgene to allow for BAC-TRAP method of RNA-isolation.