Possible mRNA targets of ZC3H11.

The Table includes all mRNAs that were at least 3-fold enriched in the ZC3H11 bound fraction, with more than 40 reads in the bound fraction and more than10 reads per million in the unbound fraction. The full lists are in Supplementary Table S1. The genes are grouped according to predicted protein function. All RNASeq results were normalised to reads per million per kilobase (rpkm) before the ratios were calculated. PC bind: Ratio of eluate to flow-through in the binding experiment. “Description” is the functional classification in TriTrypDB, with some additions and modifications. PC HS Seq: RNA rpm for procyclics heated to 41°C for 1 h, relative to procyclics kept at 27°C. PC HS RNAi: RNA read counts per million in procyclics with ZC3H11 RNAi, heated to 41°C for 1 h, divided by the read counts in wild-type procyclics heated to 41°C;*Northern blot result between 1× and 1.5× (Supplementary Figure S5);§Northern or published microarray result 5–6× [17]. BS RNAi Seq: effect of RNAi targeting ZC3H11 in bloodstream forms, relative to wild-type. “(AUU)3”: “yes” indicates the presence of (at least) the 12mer AUUAUUAUUAUU in the 3′-UTR, (yes) is a perfectly-matching 11mer. PPIase = peptidyl prolyl cis-trans isomerase; TM = trans-membrane domain, DUF866 = domain of unknown function. For multi-copy genes only one member is shown. They are HSP83 (Tb917.10.10890-10980), HSP60 (Tb927.10.6400 and 6510), and HSP70.