Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa

The resource contains experimental data collected at the Institute of Ecology and Evolution, University of Edinburgh between Jan-Oct 2023. Sixty (60) lines from the Drosophila Genetic Reference Panel (DGRP) were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed. Total number of activity events were measured for each fly individually using Drosophila Activity Monitor (DAM), measured at 1 min resolution until death. Mortality was recorded as the last detected movement in DAM. Pathogen shedding was measured for each individual fly as the number of viable P. aeruginosa colony-forming units defecated by an infected fly for 24h following oral infection.