SEMA3E-PLXND1 induced transcriptional changes in B16 melanocytes

Exposure to SEMA3E cause an immediate but transient collapse in F-actin of B16 melanocytes expressing the receptor PLXND1. Concomitant signal transduction by activated PLXND1 receptor mediated via its GTPase activating domain has a role in adapting the melanocytes to its micro-environment. To analyse these adaptive transcriptional responses B16 melanocytes were treated with SEMA3E for 2h to study early responses and for 8h to study prolonged responses. Cytochalasin D (8h) was used to distinguish the effects brough about by cytoskeletal collapse alone. Total RNA was extracted from Control, SEMA3E treated (2 hrs and 8hrs) and cytochalasin D treated (8hrs) B16 cells. The RNA was quality controlled and quantified using agilent bioanalyzer. The samples were analyzed by Agilent_mouse_GXP_8X60K array. Two biological replicates of each sample were analyzed. We acknowledge Genotypic Technology Private Limited Bangalore for the microarray processing and data analysis reported in this publication.