Wii Qi, Zhanyun Tang, Hongtao Yu (2011) CIL:35580, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset

Cultured HeLa cells were arrested in metaphase using nocodazole, immunostained for Bub1, Plk1 and CREST, and counter stained with DAPI. Images shown are maximum Z projections of convolved widefield images. Montage shows the effect of RNAi depletion of Bub1 and Plk1.

经培养的HeLa细胞在使用诺考达唑处理后被固定于有丝分裂中期，并进行了Bub1、Plk1和CREST的免疫染色，同时以DAPI进行复染。所展示的图像为卷积宽场图像的最大Z投影。拼接图展示了RNA干扰导致Bub1和Plk1耗竭的影响。