An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples
收藏资源简介:
Supplemental material and sequences obtained from the paper "An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples". <br>- Files "peerj-13885-Supplementary_table_1 and peerj-13885-Supplementary_table_2" are the supplemental material, and include the species used for cross amplification and extra sensitivity assays for the family specific primers designed.<br>- In the file "Sequences_16S_tissue" are the sequences obtained with universal primers from different fishes species used in the cross-amplification tests. The Genbank accession numbers are indicated in each sequence. <br>- In the file "Sequences_specific_primer_tissue" are the sequences obtained with the family specific primer for the five salmonids described in the paper. The Genbank accession numbers are indicated in each sequence.<br>- In the file "Sequences_specific_primer_eDNA" are the sequences obtained with the family specific primer for the environmental DNA samples of the positive controls: Nora River and El Arenero. The Genbank accession numbers are indicated in each sequence.<br><br>



