Adaptive sampling nanopore long-read sequencing of 22Rv1 cell

5-methylcytosine (5mC) is the most common chemical modification occurring on the CpG sites across the human DNA. To measure the modification, bisulfite conversion combined with short read whole genome sequencing have been established to capture the single base methylation profiling. However, the PCR amplification process could lead to duplicative methylation patterns and introduce 5mC detection bias. Additionally, the limited read length of NGS restricts co-methylation analysis between distant CpG sites. For detecting variant specific methylation, bisulfite conversion process also presents a significant challenge due to the destruction of allele information in the sequencing reads. To address these issues, we sought to characterize the human methylation profiling with the Nanopore long read sequencing data, aiming to demonstrate its potential for long-range co-methylation analysis with native modification call and intact allele information retained. To further investigate the allele specific 5mCG in prostate genome, we designed a target region covering mQTL and GWAS germline variants and generated long reads with adaptive sampling run in 22Rv1 cell line.