Effects of pharmacological manipulations of the NA system on the transcriptional stress response in the mouse hippocampus

Here, we extensively characterize noradrenaline (NA) mediated transcriptomic response during acute stress in the mouse hippocampus. Combining for the first time bulk mRNA-sequencing and selective pharmacological manipulations of the NA system. We show that the NA mediates robust stress associated transcriptomic alterations across the dorsal and ventral hippocampus via β-adrenergic receptors This repository contains data from two experiments (STR2 and STR3). First experiment (STR2): animals were injected with saline or adrenergic receptor antagonists (propranolol = beta-adrenergic receptor antagonist, prazosin = alpha-adrenergic receptor antagonist). After 45 minutes animals were then subjected either to stress, pharmacologically induced stress (Yohimbine injection) or kept in their homecage as controls. Animals were dissected 45min after onset of stress (90min after the initial injection) and dorsal hippocampus (dHC) and ventral hippocampus (vHC) were sequenced (bulk mRNA sequencing). Second experiment (STR3): Repeat of the main findings of STR3 with a better experimental design (full model with all proper control groups) and with male and female mice. Animals were injected with Propranolol (systemic beta-adrenergic antagonist), Sotalol (beta adrenergic antagonist that is unable to cross the blood brain barrier) or saline. then 45min after initial injections animals were subjected to either stress or kept in their homecage as control. 45min after onset of stress (90min after the initial injections) animals were dissected and vHC was sequenced. This repository addition contains additional data for the second experiment (STR3), adding data for a dHC for saline and propranolol injected animals