Transcription factor OsWRKY11 induces rice heading at low concentrations but inhibits rice heading at high concentrations. Transcription factor OsWRKY11 induces rice heading at low concentrations but inhibits rice heading at high concentrations

To identify direct targets of OsWRKY11 in vivo, we performed chromatin immunoprecipitation sequencing (ChIP-seq). Overall design: The ChIP assays were conducted on shoots obtained from 3-week-old OsWRKY11-OE plants. A total of 2 g of seedling shoots from transgenic pUbi:OsWRKY11-Myc plants were harvested and fixed in a crosslinking solution containing 1% (w/v) formaldehyde under vacuum for 30 min on ice. Subsequently, crosslinking was terminated by treating with 0.125 mM glycine under vacuum for 5 min.