MOESM5 of Whole genome sequencing and phylogenetic analysis of human metapneumovirus strains from Kenya and Zambia

Additional file 5: Table S2. Primer sequences used in RT-PCR for whole genome amplification. Primer nomenclature is described as follows: AF-11A1 indicates an A reaction, forward primer for amplicon 11, selected for genotype A1 (although it may function against the other 4 genotypes). Also listed are the primer sequence (5′ to 3′) the primer length, the melting temperature (calculated using the Santa Lucia algorithm (https://biopython.org/DIST/docs/api/Bio.SeqUtils.MeltingTemp-module.html) plus 3.7 C) to obtain the Breslauer values [33]) and the fraction of the primer that is G + C [34].