ATAC-sequencing of young and middle-aged SSPCs from mouse bone

Skeletal stem and progenitor cells (SSPCs) perform bone maintenance and repair. With age, they produce fewer osteoblasts and more adipocytes leading to a loss of skeletal integrity. The molecular mechanisms that underlie this detrimental transformation are largely unknown. To identify transcriptional changes that occur with aging we performed bulk RNA sequencing on young (3 month) and middle-aged (12 month) SSPCs. Overall design: Tibial and femoral SSPCs were isolated by centrifugation. Bone marrow was resuspended in growth media (GM) [DMEM containing 10% FBS and 1% penicillin/streptomycin; and then plated in 150cm2 tissue- culture flasks. Media changed the following day and then every two days. All cellular assays were performed with SSPCs at passage one from at least three different mice and young and middle-aged SSPCs for ATAC-sequencing.