Quantitative UMI-TnSeq Analysis of Acidovorax sp. KKS102, a PCB-Degrading Bacterium

Transposon mutant library sequencing using unique molecular identifiers (UMIs) for improved quantitative accuracy. A Tn mutant library of the PCB/biphenyl-degrading bacterium Acidovorax sp. KKS102 was analyzed using UMI-TnSeq before and after successive cultivation in the presence or absence of biphenyl. This approach aimed to identify mutations that enhance the fitness of this strain when grown in the presence of biphenyl.