Circular RNA profiling in gastric cancer and matched non-tumor tissues

To explore the potential role of circular RNAs in gastric cancer, we employed microarray profiling as a discovery platform. Circular RNA expression was analyzed in three paired gastric cancer and adjacent non-tumor tissues using the Arraystar Human circRNA microarray V2 (Agilent one-color). Quantile normalization and statistical testing identified circRNAs differentially expressed between tumor and non-tumor tissues, providing candidate biomarkers for gastric cancer. Circular RNA expression in gastric cancer was measured using paired tumor and adjacent non-tumor tissues. Three independent paired tissue samples were analyzed on the Arraystar Human circRNA microarray V2 (Agilent one-color).