Temperature mediated production and surface localization of BrpA.

A rabbit pre-immunization serum sample was used as a negative control (A), while hyperimmune serum generated against rBrpA was used to determine differential protein production at 22°C and 35°C (B). Immunoblots were also probed with chicken serum generated against B. turicatae rFlaB (B lower panel). Surface localization assays were performed by immunoblotting using the rabbit serum generated against rBrpA (C upper panel) and chicken serum generated against rFlaB (C lower panel). Molecular mass is depicted in kilodaltons on the left of each blot. The asterisk and arrowhead indicate the expected size of BrpA and FlaB, respectively.