SARS-CoV-2 Wastewater Data from Stockholm, Sweden

The dataset is part of a research study lead by associate professor Zeynep Cetecioglu Gurol and colleagues (KTH Royal Institute of Technology), is a collaboration between the SciLifeLab COVID-19 National Research Program and Chemical Engineering and Protein Science departments at KTH, in close collaboration with Stockholm Vatten och Avfall and the Käppala Association.The sampling of wastewater, started in mid-April 2020, from Bromma, Henriksdal, and Käppala wastewater treatment plants (WWTP). These treatment plants receive wastewater from a population of approximately 360,000; 860,000 and 500,000, respectively.After concentration, filtering, and preparation, the samples are analyzed using RT-qPCR technique for SARS CoV-2 RNA. Primers of the nucleocapsid (N) gene were used to detect the SARS-COV-2 gene (previously used and verified by Medema and colleagues (2020). In some cases, the raw wastewater has been frozen at –20 degrees, and concentrated wastewater or purified RNA have been stored at -80 C before the next analysis step was carried out. The concentration method used by prof. Zeynep Cetecioglu Gurol and her colleagues is based on their published study (Jafferali and colleagues, 2021). The gene copy nr / week is presented standardized for Bovine coronavirus (BCoV) and Pepper Mild Mottle Virus (PPMoV)For more information see https://www.covid19dataportal.se/data_types/environment/wastewater/ The dataset started week 16 2020 and updates weekly. The dataset is available as part of the Environmental Virus Profiling data section "The amount of SARS-CoV-2 virus in wastewater across Sweden" https://www.covid19dataportal.se/data_types/environment/wastewater/#stockholmon the Swedish COVID-19 Data Portal (https://covid19dataportal.se ).

本数据集隶属于由副研究员Zeynep Cetecioglu Gurol及其同事（瑞典皇家理工学院）领衔的研究项目，该项目为SciLifeLab COVID-19国家研究计划与瑞典皇家理工学院化学工程与蛋白质科学系之间的合作项目，并与斯德哥尔摩水务与废弃物处理公司及Käppala协会紧密协作。该研究始于2020年4月中旬，对Bromma、Henriksdal和Käppala污水处理厂（WWTP）的废水进行采样。这些污水处理厂分别接收约36万、86万和50万人口的废水。经过浓缩、过滤和预处理后，样品采用RT-qPCR技术对SARS CoV-2 RNA进行分析。所使用的引物为核壳（N）基因引物，用于检测SARS-COV-2基因（该引物曾由Medema及其同事（2020年）使用并验证）。在某些情况下，原始废水已冷冻保存于-20摄氏度，浓缩后的废水和纯化的RNA则存放在-80摄氏度，直至下一分析步骤。副研究员Zeynep Cetecioglu Gurol及其同事所采用之浓缩方法基于其已发表的研究（Jafferali及其同事，2021年）。基因拷贝数/周数据已按牛冠状病毒（BCoV）和辣椒花叶病毒（PPMoV）进行标准化。欲了解更多信息，请访问https://www.covid19dataportal.se/data_types/environment/wastewater/。该数据集自2020年第16周开始，每周更新。数据集作为环境病毒分析数据部分“瑞典废水中的SARS-CoV-2病毒含量”的一部分提供，可通过瑞典COVID-19数据门户（https://covid19dataportal.se）中的“斯德哥尔摩”部分获取。