Vitis vinifera Transcriptome or Gene expression. Vitis vinifera

This microarray experiment provides data which shows of the effects on gene expression from the artificial application of certain hormones on their own or in combination on ripening berries from the Adelaide Hills Various hormones have been implicated as having an effect on the grape berry ripening process. Their application prior to ripening may change the gene expression profile at veraison. The identification of the genes which are expressed at different levels due to the various hormone treatments may give insight to key gene expression changes in the ripening process. Keywords: Hormone treatment-Development Overall design: Treatments were applied one to two weeks prior to ripening and the grape berries were sampled six hours after treatment. Three biological replicates were obtained for each treatment. Treatments were applied on their own or in combination. The hormone treatments were ethephon(ey), abscisic acid(ab), brassinosteroid(br), water and aminoethoxyvinylglycine(avg), ethanol(eh) and aminoethoxyvinylglycine, abscisic acid and aminoethoxyvinylglycine, brassinosteroid and aminoethoxyvinylglycine. Control treatments were water(wa) and ethanol. Biotin labeled probes were prepared from the extracted RNA from the pooled berry samples and hybridised to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridisation station was randomised using the AlgDesign package from R.