Host-adaptation in Legionellales is 2.4 Gya, coincident with eukaryogenesis
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This dataset contains genomes, proteomes and protein alignments mentioned in Hugoson et al (2021). It has been used to analyze the evolution of host-adaptation in the order Legionellales. The data is organized by dataset type, and then by dataset. The four datasets used here are <strong>Gamma105</strong>, comprising 105 <em>Gammaproteobacteria</em> and 5 outgroups; <strong>Legio93</strong>, comprising 93 <em>Legionellales</em> and 20 outgroups; <strong>Gamma66</strong>, derived from Gamma105 by removing <em>Legionella</em>, <em>Francisella</em>, <em>Fangia</em> and <em>Piscirickettsia</em> genera; <strong>Bacteria72</strong>, comprising 49 Gammaproteobacteria and 23 outgroups. <strong>1_genomes</strong><br> Genomes as downloaded or assembled 1_1_Gamma105 1_2_Legio93 1_3_Gamma66 1_4_Bacteria72 <strong>2_proteomes</strong><br> Proteomes, as annotated by prokka 2_1_Gamma105 2_2_Legio93 2_3_Gamma66 2_4_Bacteria72 <strong>3_alignments</strong> In the first three folders, the following files are found. All sequence and alignment files are in fasta format: *_concatenated.fasta: concatenated alignment, trimmed. *.map: map of the files, tab-separated. The first row is a title row. The three first columns give the organism, the marker and the id (as found in the fasta file) for the protein. *_unaligned: non-aligned sequences for each marker. *_aligned: aligned sequences, for each marker. The prefix gives the software used for the alignment. *_trimmed: aligned, trimmed sequences for each marker. The prefix gives the software used to trim the alignment. <strong>3_1_Gamma105</strong>: Based on the Bact109 set of marker, used in Figure 5 and Supplementary Figures 2, 7 and 12 <strong>3_2_Legio93</strong>: Based on the Bact109 set of marker, used in Figure 1 and Supplementary Figures 1, 2 and 11 <strong>3_3_TB4SS_auto</strong>: Alignment of 12 genes of the T4BSS, automatically detected in all genomes. Used for the tree in Supplementary Figure 5. <strong>3_4_TB4SS_manual</strong>: Alignment of 25 genes of the T4BSS, manually curated by collinearity analysis. Used for the tree in Supplementary Figure 6. <strong>3_5_Gamma66</strong>: Based on the Bact109 set of marker, used for the tree in Supplementary Figure 9. <strong>3_6_Bacteria72</strong>: Based on the Bact109 set of marker, used for the tree in Supplementary Figure 10.



