Comparison of kinetic parameters of AP endonucleases from human and wheat.

aEach type of DNA substrates were used to measure a specific AP endonuclease activity under the appropriate optimal reaction conditions: THF•T for AP endonuclease activity, Exo20THF•G for 3′-repair phosphodiesterase activity, Exo20P•G for 3′-phosphatase activity and Exo20•G for 3′→5′ exonuclease activity (see Materials and Methods).bAPE1-catalyzed AP site cleavage and 3′→5′ exonuclease activities were measured under BER and EXO conditions, respectively. All data taken from [45].cN.D. = not determined. Kinetic parameters could not be determined because of low enzymatic activity of TaApe1L.