POLK ChIP-seq data
收藏NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP422243
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资源简介:
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for Pol ? in HEK293T cells. Overall design: Genome editing-based integration of three tandem repeats of the FLAG epitope tag (3ÃFLAG tag) into endogenous Pol ? was conducted. Then we crosslinked DNA and proteins in chromatin, fragmented DNA, immunoprecipitated endogenous Pol ? and its associated genomic DNA using anti-FLAG M2 beads, and subjected the resulting DNA fragments for next-generation sequencing analysis.
创建时间:
2024-08-10



