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ZIP-seq for functional characterizations of Z-DNA in rice

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE252954
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A Z-DNA is a left-handed DNA double helix functioning in various DNA templated events. However, antibody based high throughput methodologies for Z-DNA profiling are still missing in both humans and plants. We here developed the Z-DNA antibody-based IP-seq, termed ZIP-seq, for characterization of Z-DNA across the rice genome. Pregerminated rice seeds of Nipponbare (Oryza sativa L., Japonica) were transferred into the soil and grew in a greenhouse at 28-30℃ with a 14h/10h light-dark cycle. Two-week old seedlings were harvested and quickly frozen in liquid nitrogen for storage at -80℃ until used
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2025-04-02
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