ZIP-seq for functional characterizations of Z-DNA in rice

A Z-DNA is a left-handed DNA double helix functioning in various DNA templated events. However, antibody based high throughput methodologies for Z-DNA profiling are still missing in both humans and plants. We here developed the Z-DNA antibody-based IP-seq, termed ZIP-seq, for characterization of Z-DNA across the rice genome. Pregerminated rice seeds of Nipponbare (Oryza sativa L., Japonica) were transferred into the soil and grew in a greenhouse at 28-30℃ with a 14h/10h light-dark cycle. Two-week old seedlings were harvested and quickly frozen in liquid nitrogen for storage at -80℃ until used