H358 cells reduced representation bisulfite sequencing

In this study, using reduced representation bisulfite sequencing(RBS), we found that LSH affected DNA methylation patterns in lung adenocarcinoma (LUAD) cells. We analyzed 12,239 differentially methylated regions (DMRs) for CpG methylation and 1605 DMRs for non-CpG methylation. LSH did not change CpG methylation at a global level, but partially change the methylation of CpG islands (CGIs). Interestingly, LSH reduced CHH methylations at CGIs and consistently decreased CHG and CHH methylation patterns. However, LSH increased the CHH methylation level in satellite and simple-repeat region. The KEGG analysis showed LSH to be linked with a significant enrichment in focal adhesion and the PI3K-Akt pathway. Further analysis indicated that LSH might epigenetically regulate the binding of transcription factors, including SP2. Taken together, LSH does not affect CpG methylation but it does alter non-CpG methylation and repeat sequences in LUAD.