<i>B. nonstop</i> annotated proteins
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Protein sequences from <i>B. nonstop</i> were additionally annotated by blastp searches (with a cut-off <i>e</i>-value of 10<sup>-20</sup>) against protein datasets of a selection of kinetoplastids available from TriTrypDB (release 62) including <i>Angomonas deanei</i>, <i>Blechomonas ayalai</i>, <i>Bodo saltans</i>, <i>Crithidia fasciculata</i>, <i>Endotrypanum monterogeii</i>, <i>Leishmania major</i> Friedlin, <i>Leishmania martiniquensis</i>, <i>Leishmania mexicana</i>, <i>Leptomonas pyrrhocoris</i>, <i>Leptomonas seymouri</i>, <i>Paratrypanosoma confusum</i>, <i>Porcisia hertigi</i>, <i>Trypanosoma brucei</i> TREU927, <i>Trypanosoma cruzi</i> CL Brener Esmeraldo-like. Proteins not retrieving any hit were then screened (with a cut-off <i>e</i>-value of 10<sup>-5</sup>) against the Swiss-Prot database (downloaded February 10<sup>th</sup>, 2023) (Bairoch & Apweiler 2000, Nucleic Acids Res). Protein domains were further annotated using InterProScan v5.55-88.0 (Jones <i>et al.</i> 2014, Bioinformatics) and the Pfam database (Finn et al. 2014, Nucleic Acid Res).



