Laminar probe recordings in the mouse primary somatosensory and visual areas of awake and anesthetized mice

The goal of this experiment is to study how the activity and the connectivity between different neuronal subpopulations in the neocortex (in terms of area, layer and type of interneuron) varies as a function of brain state and sensory stimuli. The data includes electrophysiological recordings from nine mice in the mouse primary somatosensory and visual areas. These datasets comprise recordings during anesthesia (isoflurane) and awake states. In each of these states, recordings were performed under darkness and light conditions, followed by recordings with three different patterns of optogenetic stimulations. Three genetic mouse lines were used: VIP-Cre (vasoactive intestinal peptide) SST-Cre (somatostatin) and PV-Cre (parvalbumin). Viral injections were made to induce the expression of channelrhodopsin (ChR2) in the three different interneuronal subpopulations. Optogenetics was used to both identify individual neuronal subpopulations (opto-tagging) and trigger their activity to infer their role in modulating other neuronal subtypes, as a function of brain state and sensory stimulation. Recordings were performed in primary visual cortex (V1) and primary somatosensory cortex (S1HL, hindlimb area).