Preparatory metagenome assembly [Botryococcus braunii Ayame] [Freshwater green alga] [Eukaryotic phototroph and bacterial commensals] [ originally isolated from Ivory Coast, barrier lake of Ayame][Isolated by Metzger et al Phytochemistry (1988), 27, 1383-1388 ][Sequenced by Illumina Hayward][Submit

Botryococcus race B produces methylated triterpenoids which are potentially useful as biofuels. The project involved selectively assembling the orthologue and all paralogues of squalene synthase gene sequences, using Illumina data with deep (>50x) coverage, including single end reads and paired end reads. The assembly technique was based on SOAPdenovo v 1.04, with some manual curation using Sequencher for the particular contigs of interest. Paired end libraries of 2 kb and 4kb respectively were prepared by- and sequenced by- Illumina Hayward to enable crossing of introns, and hence assembly of contiguous full exons. The work was submitted to Frontiers in Microbiology, as Moore et al. 2021, and has evolutionary aims to do with assessing the order of gene duplications. In time the dataset will be curated to a higher level, and submitted to genome databases. This draft is intended to be archival and permanent

葡萄藻属菌株B（Botryococcus race B）可产生甲基化三萜类化合物，此类物质具备用作生物燃料的潜在应用价值。本项目依托覆盖度超过50×的Illumina测序数据，对靶标角鲨烯合酶（squalene synthase）基因序列的直系同源基因（orthologue）及所有旁系同源基因（paralogues）进行选择性组装，所用数据包含单端测序读段与双端测序读段。组装流程基于SOAPdenovo v1.04开发，并借助Sequencher对目标特定重叠群（contigs）开展人工精修。2 kb与4 kb的双端文库均由Illumina Hayward团队制备并完成测序，以此实现内含子的跨区域拼接，进而组装出连续完整的外显子。该项研究以Moore等（2021）的论文形式提交至《微生物学前沿》（Frontiers in Microbiology）期刊，其进化生物学研究目标为评估基因复制事件的发生先后顺序。未来本数据集将经过更高标准的整理，并提交至各类基因组数据库。本草稿旨在实现存档与永久留存。